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Figure 17A. Microglial cell-matrix interaction in 3D culture. Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 3.6 MB - MD5: f876f14728e512fd379910e9eb145f3d Representative confocal time-lapse images of 3D matrix fibers (silver) and Cx3cr1-GFP microglia (green). To explore possible microglial cell-matrix interactions in our 3D culture, we utilized a combination of fluorescent and confocal reflection microscopy techniques to visualize the interaction between microglia and the ECM. Time-lapse confocal z-s...
Figure 18A. Analysis of time-lapse confocal images of microglia in 2D and 3D culture Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 513.9 KB - MD5: da28ef81ccb86b28a85f38bff685c5c1 Representative confocal time-lapse images of microglia culture in 2D culture over 10 minutes. We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 mi...
Figure 18B. Analysis of time-lapse confocal images of microglia in 2D and 3D culture Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 194.6 KB - MD5: 3693b3f6aedba7d1152324505df772ee Representative confocal time-lapse images of microglia culture in 3D over 10 minutes. We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 min with a...
Figure 21A. Microglial phagocytic assay in 3D culture Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 17.5 MB - MD5: e8a3cc505403ef939f648cd29b7cd72b Representative confocal time-lapse images of microglia phagocytosis in 2D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads. To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The cell-matrix mixture was allowed to solidify at room temperature for 15...
Figure 21B. Microglial phagocytic assay in 3D culture Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 16.9 MB - MD5: cf58858d80c87bf778f197cecad9ca9f Representative confocal time-lapse images of microglia phagocytosis in 3D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads. To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The cell-matrix mixture was allowed to solidify at room temperature for 15...
Figure 22A. Microglia motility during phagocytosis. Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 4.3 MB - MD5: 281acbba92f54ef6ea1462c30e645691 Representative confocal time-lapse images of migrating microglia during phagocytosis for 24 hours in 2D culture. To investigate whether microglial cells migrate during phagocytosis in 3D culture, confocal z-stack time-lapse images were obtained for a period of 24 h at 15 min intervals. We observed that microglial cells migrated less during phagocyt...
Figure 22B. Microglia motility during phagocytosis. Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia' Quicktime Video - 4.2 MB - MD5: ec83c7531f9c27d54e1328692b923c5a Representative confocal time-lapse images of migrating microglia during phagocytosis for 24 hours in 3D culture. To investigate whether microglial cells migrate during phagocytosis in 3D culture, confocal z-stack time-lapse images were obtained for a period of 24 h at 15 min intervals. We observed that microglial cells migrated less during phagocyt...
Dataset: Drought damage to forest trees by hygroscopic particles Mar 7, 2025 Koch, Irmgard, 2025, "Dataset: Drought damage to forest trees by hygroscopic particles", https://doi.org/10.60507/FK2/AU4RUC, bonndata, V1 This dataset contains data from a three year PhD project concerning the drought damage to forest trees by hygroscopic paricles founded by the Umweltbundesamt in germany. From 2021 to 2024, leaves and needles from the canopies of beech, oak, spruce and pine at different level II plots of the ICP intensive forest monitoring program were sampled at di...
Koch_FeinstaubundBaueme_Readme_v1.txt Mar 7, 2025 - Dataset: Drought damage to forest trees by hygroscopic particles Plain Text - 7.3 KB - MD5: 20343f5a801bfe79fec87b5d69bc1950 README
data_FeinstaubundBaeume.csv Mar 7, 2025 - Dataset: Drought damage to forest trees by hygroscopic particles Comma Separated Values - 1.3 MB - MD5: 6752f31f7116ba8ab8671ecb36f6c61f datafile in csv format

Figure 17A. Microglial cell-matrix interaction in 3D culture.

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 3.6 MB -

Representative confocal time-lapse images of 3D matrix fibers (silver) and Cx3cr1-GFP microglia (green). To explore possible microglial cell-matrix interactions in our 3D culture, we utilized a combination of fluorescent and confocal reflection microscopy techniques to visualize the interaction between microglia and the ECM. Time-lapse confocal z-s...

Figure 18A. Analysis of time-lapse confocal images of microglia in 2D and 3D culture

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 513.9 KB -

Representative confocal time-lapse images of microglia culture in 2D culture over 10 minutes. We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 mi...

Figure 18B. Analysis of time-lapse confocal images of microglia in 2D and 3D culture

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 194.6 KB -

Representative confocal time-lapse images of microglia culture in 3D over 10 minutes. We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 min with a...

Figure 21A. Microglial phagocytic assay in 3D culture

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 17.5 MB -

Representative confocal time-lapse images of microglia phagocytosis in 2D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads. To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The cell-matrix mixture was allowed to solidify at room temperature for 15...

Figure 21B. Microglial phagocytic assay in 3D culture

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 16.9 MB -

Representative confocal time-lapse images of microglia phagocytosis in 3D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads. To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The cell-matrix mixture was allowed to solidify at room temperature for 15...

Figure 22A. Microglia motility during phagocytosis.

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 4.3 MB -

Representative confocal time-lapse images of migrating microglia during phagocytosis for 24 hours in 2D culture. To investigate whether microglial cells migrate during phagocytosis in 3D culture, confocal z-stack time-lapse images were obtained for a period of 24 h at 15 min intervals. We observed that microglial cells migrated less during phagocyt...

Figure 22B. Microglia motility during phagocytosis.

Mar 25, 2025 - Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Quicktime Video - 4.2 MB -

Representative confocal time-lapse images of migrating microglia during phagocytosis for 24 hours in 3D culture. To investigate whether microglial cells migrate during phagocytosis in 3D culture, confocal z-stack time-lapse images were obtained for a period of 24 h at 15 min intervals. We observed that microglial cells migrated less during phagocyt...

Dataset: Drought damage to forest trees by hygroscopic particles

Mar 7, 2025

Koch, Irmgard, 2025, "Dataset: Drought damage to forest trees by hygroscopic particles", https://doi.org/10.60507/FK2/AU4RUC, bonndata, V1

This dataset contains data from a three year PhD project concerning the drought damage to forest trees by hygroscopic paricles founded by the Umweltbundesamt in germany. From 2021 to 2024, leaves and needles from the canopies of beech, oak, spruce and pine at different level II plots of the ICP intensive forest monitoring program were sampled at di...

Koch_FeinstaubundBaueme_Readme_v1.txt

Mar 7, 2025 - Dataset: Drought damage to forest trees by hygroscopic particles

Plain Text - 7.3 KB -

README

data_FeinstaubundBaeume.csv

Mar 7, 2025 - Dataset: Drought damage to forest trees by hygroscopic particles

Comma Separated Values - 1.3 MB -

datafile in csv format

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