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Quicktime Video - 3.6 MB -
MD5: f876f14728e512fd379910e9eb145f3d
Representative confocal time-lapse images of 3D matrix fibers (silver) and Cx3cr1-GFP microglia (green). To explore possible microglial cell-matrix interactions in our 3D culture, we utilized a combination of fluorescent and confocal reflection microscopy techniques to visualize... |
Quicktime Video - 513.9 KB -
MD5: da28ef81ccb86b28a85f38bff685c5c1
Representative confocal time-lapse images of microglia culture in 2D culture over 10 minutes.
We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 6... |
Quicktime Video - 194.6 KB -
MD5: 3693b3f6aedba7d1152324505df772ee
Representative confocal time-lapse images of microglia culture in 3D over 10 minutes.
We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water... |
Quicktime Video - 17.5 MB -
MD5: e8a3cc505403ef939f648cd29b7cd72b
Representative confocal time-lapse images of microglia phagocytosis in 2D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads.
To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The c... |