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MD5: 960944381c08681066332072290db5b2
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Mar 25, 2025
Tambe, Bertrand Agbor, 2025, "Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'", https://doi.org/10.60507/FK2/FFPGJA, bonndata, V1
Microglia are innate immune cells of the central nervous system. They possess a diverse range of morphological features that enable them to perform different functions, such as surveillance, phagocytosis and immune response. In response to changes in physiological conditions within the CNS, microglia actively alter their shape in order to maintain... |
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MD5: e05ce1f30c6e5bf0525a5653dbcd73ad
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MD5: f876f14728e512fd379910e9eb145f3d
Representative confocal time-lapse images of 3D matrix fibers (silver) and Cx3cr1-GFP microglia (green). To explore possible microglial cell-matrix interactions in our 3D culture, we utilized a combination of fluorescent and confocal reflection microscopy techniques to visualize the interaction between microglia and the ECM. Time-lapse confocal z-s... |
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MD5: da28ef81ccb86b28a85f38bff685c5c1
Representative confocal time-lapse images of microglia culture in 2D culture over 10 minutes.
We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 mi... |
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MD5: 3693b3f6aedba7d1152324505df772ee
Representative confocal time-lapse images of microglia culture in 3D over 10 minutes.
We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 min with a... |
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MD5: e8a3cc505403ef939f648cd29b7cd72b
Representative confocal time-lapse images of microglia phagocytosis in 2D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads.
To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The cell-matrix mixture was allowed to solidify at room temperature for 15... |
Quicktime Video - 16.9 MB -
MD5: cf58858d80c87bf778f197cecad9ca9f
Representative confocal time-lapse images of microglia phagocytosis in 3D culture. Green = Cx3cr1-GFP microglia, blue = 2 um beads. To establish a microglial phagocytic assay in 3D culture, microglial cells were cultured with 2 µm polystyrene beads in Matrigel-collagen gel. The cell-matrix mixture was allowed to solidify at room temperature for 15... |
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MD5: 281acbba92f54ef6ea1462c30e645691
Representative confocal time-lapse images of migrating microglia during phagocytosis for 24 hours in 2D culture. To investigate whether microglial cells migrate during phagocytosis in 3D culture, confocal z-stack time-lapse images were obtained for a period of 24 h at 15 min intervals. We observed that microglial cells migrated less during phagocyt... |
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MD5: ec83c7531f9c27d54e1328692b923c5a
Representative confocal time-lapse images of migrating microglia during phagocytosis for 24 hours in 3D culture. To investigate whether microglial cells migrate during phagocytosis in 3D culture, confocal z-stack time-lapse images were obtained for a period of 24 h at 15 min intervals. We observed that microglial cells migrated less during phagocyt... |