Microscopic analysis of human ASC-derived kidney organoids for polycystic kidney disease modelling

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Microscopic analysis of human ASC-derived kidney organoids for polycystic kidney disease modelling
Ashraf, Arsila Palliyulla Kariat; Rohe, Jana, 2026, "Microscopic analysis of human ASC-derived kidney organoids for polycystic kidney disease modelling", https://doi.org/10.60507/FK2/OM25XQ, bonndata, V1
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Description
Polycystic kidney disease (PKD) is a genetic disorder and a major contributor to end-stage renal disease. Elucidating the cellular mechanisms underlying PKD necessitates three-dimensional models that accurately replicate cystogenesis in vitro. Existing organoid models frequently exhibit limited expression of adult kidney epithelial markers and lack standardized methods for cyst identification. This microscopy imaging data deposit supports our efforts to develop and characterize a model that more faithfully reflects adult kidney epithelium and provides a robust read-out for PKD research. We generated adult stem cell (ASC)-derived human kidney organoids (tubuloids) from patient tissue, and cultured them either in suspension or embedded in extracellular matrix (domes). This approach allowed us to compare organoid structure, cell polarity, cellular composition, and the representation of kidney epithelium during organoid development. Using pharmacological stimulation and tissue imaging, we demonstrate that these tubuloids reproducibly form cysts in vitro upon chronic cAMP signaling, making these data valuable for PKD investigation. Microscopy datasets (image and video files) of fixed and live tubuloids are presented, analyzed for cyst formation, expression of specific proteins of interest, and transduction efficiency. (2025-04-15)
Subject Medicine, Health and Life Sciences
Related Publication IsSupplementTo: Rohe, J., Ashraf, A.P.K., Kehl, M. et al. Modelling kidney cystogenesis using human kidney tubuloid cultures. BMC Mol and Cell Biol 27, 26 (2026). https://doi.org/10.1186/s12860-026-00591-xdoi: 10.1186/s12860-026-00591-x
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README_Rohe-Ashraf-et-al_BMC_Microscopic-analysis-of-human-ASC-derived-kidney-organoids-for-polycystic-kidney-disease-modelling.txt
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Published May 12, 2026
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MD5: 5e5b83701e49ccb9b33ea26bd8f48d97
Readme file with information on the image datasets.
Preview "README_Rohe-Ashraf-et-al_BMC_Microscopic-analysis-of-human-ASC-derived-kidney-organoids-for-polycystic-kidney-disease-modelling.txt"
Rohe-Ashraf-et-al-2025-BMC_Figure-1_Dome-and-Suspension-Tubuloids.zip
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Published May 12, 2026
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MD5: 6d9ac2c44fc8c5cb5e0abfef14c6b374
Bright-field microscopy images of tubuloids in dome and suspension culture from passages P0 to P5.
Rohe-Ashraf-et-al-2025-BMC_Figure-2_Immunohistochemistry.zip
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Published May 12, 2026
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MD5: e31532faa580d8881c322183218f8f65
Bright-field microscopy images of dome and suspension tubuloids at P1 and P3, stained for CK7, CK19 and PAX8. Bright-field microscopy images of dome and suspension tubuloids at P1 and P4, stained for GATA3, CDH1 and CD13.
Rohe-Ashraf-et-al-2025-BMC_Figure-3_Immunohistochemistry.zip
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Published May 12, 2026
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Bright-field microscopy images of dome and suspension tubuloids at P1 and P3/P4, stained for CD24, ATP1A1, CDH16 and TACSTD2.
Rohe-Ashraf-et-al-2025-BMC_Figure-4_Cyst-Induction.zip
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MD5: 89dbdd5be4b3d4c4602536f35bca3f70
Bright-field microscopy images of Forskolin, DMSO or untreated dome and suspension tubuloids used for the quantification of cyst induction.
Rohe-Ashraf-et-al-2025-BMC_Figure-5_Immunostaining.zip
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Immunofluorescence images of Forskolin, DMSO or untreated dome and suspension tubuloids used for the quantification of tubuloid epithelial width and ciliary length.
Rohe-Ashraf-et-al-2025-BMC_Figure-S1_Dome-and-Suspension-culture_MIB1.zip
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MD5: 0572bbc7020d9fa3ba69169e454ce914
Bright-field microscopy images of live dome tubuloids at P6 to P8 and primary human kidney cells immediately cultured in suspension after tissue dissociation. Microscopy images and slide scans of fixed dome and suspension tubuloids at P1, stained for MIB1.
Rohe-Ashraf-et-al-2025-BMC_Figure-S3B_GATA3-quantification.zip
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Microscopy images and slide scans of fixed dome and suspension tubuloids in P1 and P4, stained for GATA3 and used for quantification.
Rohe-Ashraf-et-al-2025-BMC_Figure-S5_Transduction.zip
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MD5: d9e7c5a71221c4f871cb7841ba2404db
Immunofluorescence images of rAAV2/8-CMV-EGFP transduced dome and suspension tubuloids at P3D10.
Rohe-Ashraf-et-al-2025-BMC_Live-cell-imaging.zip
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MD5: a2268a907153af028653f55cb1b81406
Phase-contrast and fluorescent microscopy images of live suspension tubuloids at P3, stained with Calcein-AM.
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