Clinical dataset on monocyte cholesterol crystal uptake and cyclodextrin exposure in patients undergoing coronary angiography

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Luebbering, Nikola; Zimmer, Sebastian, 2025, "Clinical dataset on monocyte cholesterol crystal uptake and cyclodextrin exposure in patients undergoing coronary angiography", https://doi.org/10.60507/FK2/0SYROH, bonndata, V1
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Description
This observational study includes patients undergoing diagnostic coronary angiography. For each participant we document demographic characteristics, cardiovascular risk factors, angiographic findings (presence and extent of CAD, PCI), laboratory markers of inflammation and metabolism, and medication at the time of the study. In addition, we assess cholesterol crystal (CC) uptake by circulating monocytes and the corresponding cyclodextrin (CD)-related effect (ΔCC) as imaging-/signal–derived measures. The overall purpose is to characterize this patient cohort and to examine whether CC uptake and its change are related to the burden of coronary artery disease and to clinically relevant parameters such as hypertension, diabetes mellitus, lipid profile, inflammatory markers (e.g. CRP, leukocytes) and current cardiovascular medication.
Subject Medicine, Health and Life Sciences
Related Publication IsSupplementTo: Lübbering N, Krogmann A, Jansen F, Latz E, Nickenig G, Zimmer S (2025) Cyclodextrin reduces cholesterol crystal uptake by circulating monocytes in patients undergoing coronary angiography. PLoS One 20(12): e0338635.doi: 10.1371/journal.pone.0338635
Notes Sample collection protocol: Whole blood samples were collected from 76 patients undergoing coronary angiography Sample preparation protocol: For separation of peripheral blood mononuclear cells (PBMC), a cell separation medium with a polyester gel and a density gradient liquid was used. PBMC were dissolved in the patient`s own serum (7.2 ml) and diluted in (7.8 ml) phosphate-buffered saline (PBS). Analysis protocol: The PBMC were preincubated with 2-Hydroxypropyl-gamma-CD (10 mM) or PBS control for 6h at 37°C and subsequently incubated with CC (120 x 106 particles) for 30 min at 37°C. CC-uptake by monocytes was evaluated and quantified by flow cytometry. After stimulation with CD and CC, PBMC were treated with 12.5 µl 4 % paraformaldehyde in PBS. To differentiate monocytes from lymphocytes and cell debris, we used three different monoclonal antibodies: PE anti-human CD11b (Clone ICRF44, BioLegend, San Diego, CA, USA), FITC anti-human CD14 (Clone M5E2, BioLegend) und PerCP anti-human CD45 (Clone 2D1, BioLegend) (Dilution: 1 µg/test). Methods for processing the data: CC uptake was measured using the protocol described above. The CCΔCD value was calculated as the difference between CC uptake without CD and CC uptake with CD.
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