bonndata catalog
Thumbnail for The Diagonal Band of Broca Regulates Olfactory-mediated Social Behaviors: A Study Using USVs As Unbiased, Ethological Markers For Social Interactions

The Diagonal Band of Broca Regulates Olfactory-mediated Social Behaviors: A Study Using USVs As Unbiased, Ethological Markers For Social Interactions

Open this dataset in the live repository

Important usage condition: Use and redistribution are governed by CC BY 4.0. Review and comply with the license before using the data.
Persistent identifier
doi:10.60507/FK2/WB6DOI
Published version
1.1
Publication date
2025-08-14
License
CC BY 4.0

Description

The Horizontal Diagonal Band of Broca (HDB) modulates olfactory information processing by recruiting olfactory bulb (OB) interneurons to shape excitatory OB output. Here, we show that OB is strongly and differentially innervated by HDB projections. Light-induced silencing of HDB afferents in the OB via OPN3 affected olfactory-mediated social habituation and discrimination behaviors, as shown by manual annotation of investigative behavior. Additionally, we established ultrasonic vocalizations (USVs) as an unbiased, reliable, and ethologically relevant biomarker for quantifying investigative social behavior. To this end, we employed "DeepFisFis," an AI-based solution, developed in our lab for detection of mouse USVs. Collectively, our studies demonstrate that USVs serve as an effective biomarker for investigative social behavior. Furthermore, our results highlight the HDB as a crucial top-down relay in the modulation of olfactory-mediated social behaviors.

Creators

Keywords

Horizontal Diagonal Band of Broca, Olfactory bulb, Ultrasonic vocalizations, Optogenetic silencing

Files

Before downloading: Use and redistribution are governed by CC BY 4.0. Review and comply with the license before using the data.
FileTypeBytesChecksum
16756_SIT_3.h5application/x-h5300336MD5 3ad34117386e00ce1e53317fb9ed6418
16727_SIT_4.h5application/x-h5347407MD5 4746b6dd0ec450f41db2d3951d8615b8
16888_SIT_4.h5application/x-h5311153MD5 79f218e97456e96b6aea99f987d1943f
16887_SIT_trials_borders.csvtext/csv238MD5 ccb1db89699e09ab7c1e6e0d49d1811f
16939_SIT_0.h5application/x-h5349791MD5 8f6c72802d66236b8ae35819655588a5
16727_SIT_3.h5application/x-h5349897MD5 8d1c41e311df462cf72b877e641cdd8e
16887_SIT_4.h5application/x-h5299268MD5 3ea0749be404253a3d64ac7a1125f813
16726_SIT_3.h5application/x-h5350262MD5 3d83395ef25f7ead52fcaab1c7e9729f
17098_SIT_2.h5application/x-h5289183MD5 c5d4b54d17a7d57df37fb178883e090b
16755_SIT.avivideo/x-msvideo1925738016MD5 2a2fa1b27905280ba2c7204a4cb9f8eb
16754_SIT_1.h5application/x-h5333650MD5 e03ca89de1a445eb6c93f1c2b266187b
16753_SIT.avivideo/x-msvideo1945606074MD5 2740702fb168e7b599bc12810788ef71
16889_SIT_0.h5application/x-h5312135MD5 1915f34f789d29cf2c97393dfa4e2b66
16753_SIT_USV_intervals.csvtext/csv4080MD5 3b6658eb066320123cf7041df8c1266a
16726_SIT_0.h5application/x-h5358130MD5 97d637401d3d527bb306f3910d3b48f4
17098_SIT.avivideo/x-msvideo1889893152MD5 9ad50acc3f5f64515ac677bfac5a0b91
16889_SIT.avivideo/x-msvideo1924900388MD5 a93207e538b43631be15577f8f32178b
16726_SIT_4.h5application/x-h5340982MD5 07b6a4f761d622a4208f412b9e221d62
16889_SIT_2.h5application/x-h5305730MD5 ebe6858cacff27c9c525425dd2f1db81
16939_SIT_3.h5application/x-h5349811MD5 e24a6ebca7dfc92d3c46130e30163757
17096_SIT_2.h5application/x-h5308084MD5 0e83e5726d57f4096c015846132617c9
16889_SIT.wavaudio/wav544711724MD5 ddd93738b53b9ff6c77dbdac25c862d8
16727_SIT_1.h5application/x-h5353811MD5 7ced7b701116a331082be4bce3bed023
17096_SIT_4.h5application/x-h5309662MD5 270c6c5589a444d9077aff325dba9dd3
16889_SIT_5.h5application/x-h5311605MD5 9eca677c6cda4334184161678b2f35d8
16755_SIT_trials_borders.csvtext/csv239MD5 df77c1e6e997bcad75a97732ecd11b2d
16757_SIT.wavaudio/wav520558124MD5 00d54d2ad628bd2ae1592e49e528548a
17095_SIT_3.h5application/x-h5298613MD5 772f1c1e5fc35c7e2131ba4c8a90e1ec
16890_SIT_USV_intervals.csvtext/csv21473MD5 7dcd2ab9b675c463207cdb2b813eec40
16727_SIT.avivideo/x-msvideo2330978206MD5 7b2eb745fada7ed81f57a1a677aa8346
16889_SIT_trials_borders.csvtext/csv237MD5 876a16381340c6ddeb2ac7a4e07f57a1
16757_SIT_USV_intervals.csvtext/csv45807MD5 605d1d30a1222710efd969e60a4a4416
17098_SIT_4.h5application/x-h5303903MD5 f1d39cda824ff73fd22254123a4c5df8
16757_SIT_2.h5application/x-h5352732MD5 464e38d6f48a757240f952abc7b5457a
17095_SIT_0.h5application/x-h5312332MD5 ae3a94e67045ffb9ca7025e1f21b136f
16727_SIT_trials_borders.csvtext/csv237MD5 adb3f3ac835fb16a715326b6c6f0c6b4
16939_SIT_4.h5application/x-h5362293MD5 7060d0d81f1fec6e853b9b6e76a0e23e
16727_SIT.wavaudio/wav543283244MD5 12f89dad0d98c5a920836e01139fa880
16889_SIT_4.h5application/x-h5305963MD5 3923933e8a0a6d7dd4346fc16b6b2bf5
16755_SIT_USV_intervals.csvtext/csv3276MD5 6506a14eef846ddea32a8014b4821cbb
17095_SIT_4.h5application/x-h5301945MD5 81bf477bf3e1e887b224ac7489e82326
16753_SIT_1.h5application/x-h5310785MD5 3fa1f7115208688619adb9c8f31f013a
16727_SIT_USV_intervals.csvtext/csv16508MD5 635d5c015d132f8ef00890223fa7344e
16726_SIT_USV_intervals.csvtext/csv31441MD5 4f2b8edf98984a0db535eea04d1a1f47
16757_SIT_trials_borders.csvtext/csv236MD5 24822f01b99987220706fff4fc3b8711
16757_SIT_0.h5application/x-h5357783MD5 8c87591323414f0729d49e7609db564b
16890_SIT_2.h5application/x-h5311600MD5 d2b8066bb9ff599680abd44983ba9536
17095_SIT_5.h5application/x-h5313250MD5 0a284758e553201f18c01491b1decbb7
16939_SIT_2.h5application/x-h5353344MD5 f3adecbfefac5ade48f4c7f8befaba6a
16939_SIT_5.h5application/x-h5370399MD5 90e9f8b50cc794d1e4d18434f0e0d02e
16888_SIT_3.h5application/x-h5304285MD5 f62f79f089238e0e312f392e7e79eb7d
16755_SIT_4.h5application/x-h5281222MD5 601c6226b0c5a02b02107f7949fa7bcc
16890_SIT_0.h5application/x-h5310489MD5 ababbfff11d802c124f665854d0674d4
16757_SIT_4.h5application/x-h5347681MD5 94304020b1c7049186d6b7f4620b860f
17095_SIT_2.h5application/x-h5307780MD5 dc6a43e56cc7967734527e3cc206d874
16755_SIT_5.h5application/x-h5310519MD5 f5d8c9f3085a11ff09836d8f0c1aee45
16887_SIT.avivideo/x-msvideo1905594154MD5 b8626aeef225f17988281432dec04604
17098_SIT_0.h5application/x-h5304559MD5 4e6c8c19073de0aa01cba06c2f141de6
16939_SIT_USV_intervals.csvtext/csv8146MD5 61e8ca7223f22f053c223c71e04e6316
17095_SIT_trials_borders.csvtext/csv236MD5 0af152943ed56955246a882b9bfdf9d4
16888_SIT_trials_borders.csvtext/csv238MD5 3c72545c8a9547f1b25cfec9bf6a66e4
16754_SIT_2.h5application/x-h5319761MD5 a0b44864f3f8cbf774aff4383f0c3555
17096_SIT.wavaudio/wav497694764MD5 668023db07c0c29e97ddcb894a9e380c
16887_SIT.wavaudio/wav529443884MD5 0b4fd79697a6b2e96584fbceb6bdfae6
17096_SIT_0.h5application/x-h5300564MD5 0b822c0a2da7a1d251bf0e7ab8c68c60
17095_SIT.wavaudio/wav578465324MD5 ed8d4e73c9735e131803f8d788f2f14a
16753_SIT_4.h5application/x-h5295503MD5 aba28e1a6e2d5c5eac5eb3fdaffeec6a
16888_SIT_0.h5application/x-h5307377MD5 882c9881398020743f60c3f5bed78c60
16939_SIT_trials_borders.csvtext/csv241MD5 bfee46354ad3c4512bde38ab339d99b7
17095_SIT_1.h5application/x-h5306541MD5 5d57a5485cb6592bc70d9f21a3e2cbba
16889_SIT_3.h5application/x-h5309758MD5 ab6eff26d4646d70e77d9f6361526d57
16757_SIT_5.h5application/x-h5357067MD5 1fe031e9e85449774b403412a1f9a994
16753_SIT.wavaudio/wav543260204MD5 f95e705905c2edce3fb304b2ff9bb399
17096_SIT_trials_borders.csvtext/csv243MD5 5de3444c5d8a9a990d87b1e65787e4ca
Mohammadi_OB-OPN3_Readme.txttext/plain3389MD5 0930237c42c0eda20b5f9f2d47c6c7d9
16754_SIT_0.h5application/x-h5355991MD5 0a2d06ea9ec073c9edd8a33bb4c5bbf9
16756_SIT_1.h5application/x-h5296134MD5 17dea28a4f02da187fd55b503b61e7a3
16757_SIT_3.h5application/x-h5347493MD5 45f342237a685539a075156374eea3c6
16755_SIT_2.h5application/x-h5290041MD5 bb7134e4144f593ffef0edc7a0c43d6c
16756_SIT.avivideo/x-msvideo1941189376MD5 395a2ef3828b48453eff34bd2e5691e1
16754_SIT_5.h5application/x-h5364932MD5 c0e6269922dfbf40230fef30bdf0dc3a
16754_SIT_4.h5application/x-h5342789MD5 e3161f6638d95846e3801d736bc403c7
16939_SIT.wavaudio/wav533030444MD5 4e29a84d149816831bc7a28a54bb24bb
16756_SIT_4.h5application/x-h5295554MD5 bbcbc6de9b3d51950ebac06ba3e24a01
16753_SIT_2.h5application/x-h5303581MD5 fd318fca851274df6a2cdb7672c9eab6
16889_SIT_USV_intervals.csvtext/csv11857MD5 f996ff4a398b0820d868266ba55b9fec
16889_SIT_1.h5application/x-h5308369MD5 adf87a25f0819b15f04b972d81233fc2
16756_SIT_USV_intervals.csvtext/csv33072MD5 257530d940b7da02154d27c1e1534b67
16754_SIT_trials_borders.csvtext/csv239MD5 d701b1b2cbbc4aee65ebe6c21278cd03
17095_SIT_USV_intervals.csvtext/csv53250MD5 826375ce998fbafda484aff5e7c70c74
16754_SIT.avivideo/x-msvideo2144701736MD5 eecfd0d5ce00180e44d44677dc98703a
17098_SIT.wavaudio/wav528207404MD5 298da23d24d9d313f74f690260db2617
16756_SIT.wavaudio/wav557184044MD5 6c9f5de908444d1bea0a58e3ccfbcd63
16890_SIT_4.h5application/x-h5279107MD5 da425426187e578de999fc34ac3ec271
17096_SIT.avivideo/x-msvideo1668126798MD5 898c4e0aa789278a75331c3155ce0dfb
17096_SIT_3.h5application/x-h5312167MD5 a34529e2f96cbcac2720b37994eb02d2
16890_SIT.wavaudio/wav556899884MD5 ca2bf5e05f582613dd7ab14b2a51cd9d
16726_SIT_2.h5application/x-h5342632MD5 5cbea930245cf93770d2ad5eb4b80552
16753_SIT_3.h5application/x-h5287807MD5 35d19eeb7eea5a425f5808e1dfad5211
16939_SIT_1.h5application/x-h5350414MD5 f354ca4f83d0d243e6e6f7d0fabd1a89
16888_SIT_USV_intervals.csvtext/csv35146MD5 65bdf9bf9f40c9ed38a5931725dcb9a9
16888_SIT.wavaudio/wav525319724MD5 e3795aef72e32430d969c5d96cae29d5
16756_SIT_5.h5application/x-h5305475MD5 37edc416b3cf19dcafd787173329c0af
16887_SIT_5.h5application/x-h5305370MD5 9ac94181b456b0a1db29174edbe11f07
16756_SIT_2.h5application/x-h5298725MD5 700e0eac75b331568be469aafe53456f
16755_SIT_1.h5application/x-h5296486MD5 59cae856f23a550ae568f655e0413279
16754_SIT_3.h5application/x-h5327312MD5 c2838b0ed37c6f9be813588c1c7b12a7
16755_SIT.wavaudio/wav540533804MD5 75dbedeb5348c99008a521efeb1be010
16753_SIT_trials_borders.csvtext/csv244MD5 f2bb5f3b428e09d5ceda8c713378f3c3
16754_SIT.wavaudio/wav510919724MD5 97bbcb942c6b02beff8cf63a812c112d
16726_SIT.wavaudio/wav524666924MD5 4f115a269b7640807f0bab489faf611b
17096_SIT_USV_intervals.csvtext/csv37172MD5 8a0cc62f90b0101896d930a88be316cd
17098_SIT_1.h5application/x-h5293111MD5 d84efc223e51316808661d8d53e95e63
16890_SIT_5.h5application/x-h5312356MD5 73841178739e8a75a1b5d8ebf8019e67
16756_SIT_trials_borders.csvtext/csv238MD5 006f4fdefc0bfcc05e707278beec374d
16753_SIT_5.h5application/x-h5299611MD5 3445bff43acc9d97e6a42cfda3fc0911
17098_SIT_USV_intervals.csvtext/csv37503MD5 0b2ede21a1dddc3323bec2a80cad5c47
16756_SIT_0.h5application/x-h5297752MD5 5ce1295e75b595ea45a31798e2dacffd
16727_SIT_0.h5application/x-h5357039MD5 1acb1476d2beceee6edeb12ab92158da
17098_SIT_5.h5application/x-h5309118MD5 c08688691535c57d8a1783c3a13dc719
16753_SIT_0.h5application/x-h5305734MD5 a43dce0eda6f10287607e6beb11b71e2
16888_SIT_5.h5application/x-h5298174MD5 e74b15ff4e74ac0c9ed5a41334ceb256
17096_SIT_5.h5application/x-h5310812MD5 2b82ddb65a8776fbb5b74b8bcfbbc95a
16887_SIT_0.h5application/x-h5309640MD5 790787e43277d4af08c31eb90ce30cf1
16888_SIT_2.h5application/x-h5307388MD5 562a51a0fd207d7212e2061edd736b5c
16890_SIT_trials_borders.csvtext/csv240MD5 5fe0e4aee3b29cd5b128ca2ed558b253
16754_SIT_USV_intervals.csvtext/csv26955MD5 3ea9e7a9d59244ab033b305e65480bc9
17098_SIT_trials_borders.csvtext/csv241MD5 ec6e92e93a0a74cd872eec8eb7a89404
16726_SIT_trials_borders.csvtext/csv242MD5 d21f4064870d8793f8646fa8a8933ddf
16757_SIT_1.h5application/x-h5360409MD5 ffba48642f64929f35203a7aba69d185
16726_SIT.avivideo/x-msvideo2161213224MD5 b1c186385da4856021f40b5f823b2bcb
16755_SIT_0.h5application/x-h5303749MD5 47739484b984d8271fec0d395208a5b1
17096_SIT_1.h5application/x-h5307460MD5 a7c1612639250bae88b54592909b0de1
16757_SIT.avivideo/x-msvideo2147869100MD5 a647924a4af35dcc8ab9c280f4526cff
16887_SIT_1.h5application/x-h5308648MD5 02292945b145ba7e9a2b4aa9121b4439
16890_SIT_1.h5application/x-h5309264MD5 995f4ecc5395e2525c18801b0a1fe2ee
16890_SIT_3.h5application/x-h5308218MD5 58a6654e483e540a79377647625f8d39
16939_SIT.avivideo/x-msvideo2221093004MD5 5e5a861719d970b731967347f4c6482a
16887_SIT_3.h5application/x-h5306500MD5 61761c5d1d93368b4d3e4b79c72cf584
16887_SIT_USV_intervals.csvtext/csv423MD5 81ead70c7057b47fef32d73b4cd80705
16888_SIT_1.h5application/x-h5304251MD5 99e5ce50cbf6230aeb795963fdb6e0dc
16888_SIT.avivideo/x-msvideo1863611236MD5 6b2486f97cb6dfdb089fed08b871c151
16727_SIT_2.h5application/x-h5357796MD5 ea6be5a6124acd75c44abf854de82d50
16890_SIT.avivideo/x-msvideo1970690886MD5 2c3c6ef79eac425049c52d9a49dcaa2e
16755_SIT_3.h5application/x-h5299602MD5 512ec73b937a1c16ae49b3212fe41412
16726_SIT_5.h5application/x-h5361629MD5 e74a90927135429506140e8c3e280072
16726_SIT_1.h5application/x-h5347831MD5 fcbf31f1477e5e08c3edae24803a036c
16727_SIT_5.h5application/x-h5361690MD5 34bbc2148a59a7b0f2f55d3064a18d3b
17095_SIT.avivideo/x-msvideo2033513052MD5 02067baa73a3a6d32043b67612fc117e
17098_SIT_3.h5application/x-h5304728MD5 8c5e7036d926dcfaa94fed6c5c0a5888
16887_SIT_2.h5application/x-h5301191MD5 2b40f5c564ba24d1b030e7f704a62214

Citation

Mohammadi, Ali; Fuhrmann, Falko; Tillmann, Jens F.; Fuhrmann, Martin; Schwarz, Martin K., 2025-08-14, The Diagonal Band of Broca Regulates Olfactory-mediated Social Behaviors: A Study Using USVs As Unbiased, Ethological Markers For Social Interactions, doi:10.60507/FK2/WB6DOI, V1.1

Additional Dataverse fields

Id471
Dataset Typedataset
Internal Version Number5
Latest Version Publishing StateRELEASED
Release Time2025-08-28T11:36:40Z
Create Time2025-08-16T20:38:56Z
Citation Date2025-08-14
File Access RequestTrue

Export metadata

Static metadata exports available for this published dataset version:

Complete Dataverse metadata

Citation Metadata

Title title
The Diagonal Band of Broca Regulates Olfactory-mediated Social Behaviors: A Study Using USVs As Unbiased, Ethological Markers For Social Interactions
Author author
  1. Author Name
    Mohammadi, Ali
    Author Affiliation
    University of Bonn
  2. Author Name
    Fuhrmann, Falko
    Author Affiliation
    Deutsches Zentrum für Neurodegenerative Erkrankungen e.V.
  3. Author Name
    Tillmann, Jens F.
    Author Affiliation
    University of Bonn
  4. Author Name
    Fuhrmann, Martin
    Author Affiliation
    Deutsches Zentrum für Neurodegenerative Erkrankungen e.V.
  5. Author Name
    Schwarz, Martin K.
    Author Affiliation
    University of Bonn
Dataset Contact datasetContact
  1. Dataset Contact Name
    Schwarz, Martin K.
    Dataset Contact Affiliation
    University of Bonn
    Dataset Contact Email
    Martin.Schwarz@ukbonn.de
Ds Description dsDescription
  1. Ds Description Value
    The Horizontal Diagonal Band of Broca (HDB) modulates olfactory information processing by recruiting olfactory bulb (OB) interneurons to shape excitatory OB output. Here, we show that OB is strongly and differentially innervated by HDB projections. Light-induced silencing of HDB afferents in the OB via OPN3 affected olfactory-mediated social habituation and discrimination behaviors, as shown by manual annotation of investigative behavior. Additionally, we established ultrasonic vocalizations (USVs) as an unbiased, reliable, and ethologically relevant biomarker for quantifying investigative social behavior. To this end, we employed "DeepFisFis," an AI-based solution, developed in our lab for detection of mouse USVs. Collectively, our studies demonstrate that USVs serve as an effective biomarker for investigative social behavior. Furthermore, our results highlight the HDB as a crucial top-down relay in the modulation of olfactory-mediated social behaviors.
Subject subject
  1. Medicine, Health and Life Sciences
Free Keyword freeKeyword
  1. Free Keyword Value
    Horizontal Diagonal Band of Broca
  2. Free Keyword Value
    Olfactory bulb
  3. Free Keyword Value
    Ultrasonic vocalizations
  4. Free Keyword Value
    Optogenetic silencing
Publication publication
  1. Publication Relation Type
    IsSupplementTo
    Publication Citation
    Schwarz, I. et al. The diagonal band of Broca regulates olfactory-mediated behaviours by modulating odor-evoked responses within the olfactory bulb. bioRxiv 2020.11.07.372649 (2020). https://doi.org/10.1101/2020.11.07.372649
    Publication IDType
    doi
    Publication IDNumber
    10.1101/2020.11.07.372649
    Publication URL
    https://doi.org/10.1101/2020.11.07.37264
Depositor depositor
Mohammadi, Ali
Date Of Deposit dateOfDeposit
2025-08-13

Expected crawler behaviour

Use a stable, truthful User-Agent with product/version and a working contact URL. Across all IP addresses and HTTP connections used by one crawler identity, allow no more than 5 requests in flight and wait at least 20 seconds between request starts. Crawl URLs listed in the catalog sitemap, including file pages and download URLs when they are published, use conditional requests, honor Retry-After, and apply exponential backoff after errors.

The welcome page may link to the interactive repository for human navigation. Automated clients must not treat that human link as a catalog crawl target.

Read the live machine-readable crawler policy before and during a crawl. Stop crawling when it reports CPU or memory utilization at or above 80% and 80% respectively.