Image data related to the publication "Kupffer cell programming by maternal obesity triggers fatty liver disease"
type: Dataset
creator: Huang, Hao
creator: Mass, Elvira
author: Huang, Hao
author: Mass, Elvira
date published: 2025-04-03
date modified: 2025-04-03
version: 1
description: Kupffer cells (KCs) are tissue-resident macrophages which colonize the liver early during embryogenesis. Upon liver colonization, KCs rapidly acquire a tissue-specific transcriptional signature, mature alongside the developing liver, and adapt to its functions. Throughout development and adulthood, KCs perform distinct core functions essential for liver and organismal homeostasis, including supporting fetal erythropoiesis, postnatal erythrocyte recycling, and liver metabolism. However, whether perturbations of macrophage core functions during development contribute to or cause disease at postnatal stages is poorly understood. Here, we utilize a mouse model of maternal obesity to perturb KC functions during gestation. We show that offspring exposed to maternal obesity develop fatty liver disease, driven by aberrant developmental programming of KCs that persists into adulthood. Programmed KCs promote lipid uptake by hepatocytes through apolipoprotein secretion. KC depletion in neonates born to obese mothers, followed by replenishment with naïve monocytes, rescues fatty liver disease. Further, genetic ablation of hypoxia-inducible factor alpha (Hif1a) in macrophages during gestation prevents the metabolic programming of KCs from oxidative phosphorylation to glycolysis, thereby averting the development of fatty liver disease. These results establish developmental perturbation of KC functions as a causal factor in fatty liver disease in adulthood and position fetal-derived macrophages as critical intergenerational messengers within the concept of developmental origins of health and diseases.
keywords: Medicine, Health and Life Sciences
citation: Huang, H., Balzer, N.R., Seep, L. et al. Kupffer cell programming by maternal obesity triggers fatty liver disease. Nature 644, 790–798 (2025).
license: http://creativecommons.org/licenses/by/4.0
included in data catalog: bonndata
publisher: bonndata
provider: bonndata
funder: DFG, German Research Foundation
distribution: Hepatocyte_lipid_accumulation_cultured_with_KCs.zip
distribution: Hepatocyte_lipid_accumulation_with_factors.zip
distribution: Hif1a_P0_liver_macs_HFD_or_CD-fed_dams.zip
distribution: Maternal_Obesity_Oil-red-O_Clec4f-DTR_cohort.zip
distribution: Maternal_Obesity_Oil-red-O_Hif1a_cohort.zip
distribution: Maternal_Obesity_Oil-red-O_WT_cohort.zip
distribution: Readme_Huang_Image_Data_Liver_Maternal_Obesity.rtf
distribution: Readme_Huang_Image_Data_Liver_Maternal_Obesity.txt

Huang, Hao
type: Person
given name: Hao
family name: Huang
affiliation: University of Bonn

Mass, Elvira
type: Person
given name: Elvira
family name: Mass
affiliation: University of Bonn

Huang, H., Balzer, N.R., Seep, L. et al. Kupffer cell programming by maternal obesity triggers fatty liver disease. Nature 644, 790–798 (2025).
type: CreativeWork

Hepatocyte_lipid_accumulation_cultured_with_KCs.zip
type: DataDownload
encoding format: application/zip
content size: 684198122
description: Data were acquired with Incucyte (Sartorius). The lipid accumulation is indicated by the LD540 signal. KCs are Kupffer cells.

Hepatocyte_lipid_accumulation_with_factors.zip
type: DataDownload
encoding format: application/zip
content size: 441075593
description: Data were acquired with Incucyte (Sartorius). The lipid accumulation is indicated by the LD540 signal. Factors used were: TNFa or ApoE or ApoA1.

Hif1a_P0_liver_macs_HFD_or_CD-fed_dams.zip
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encoding format: application/zip
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description: macs are macrophages

Maternal_Obesity_Oil-red-O_Clec4f-DTR_cohort.zip
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Maternal_Obesity_Oil-red-O_Hif1a_cohort.zip
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Maternal_Obesity_Oil-red-O_WT_cohort.zip
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encoding format: application/zip
content size: 913753640

Readme_Huang_Image_Data_Liver_Maternal_Obesity.rtf
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encoding format: text/rtf
content size: 10144

Readme_Huang_Image_Data_Liver_Maternal_Obesity.txt
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encoding format: text/plain
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