{"id":11321,"identifier":"FK2/OM25XQ","persistentUrl":"https://doi.org/10.60507/FK2/OM25XQ","protocol":"doi","authority":"10.60507","separator":"/","publisher":"bonndata","publicationDate":"2026-05-12","storageIdentifier":"file://10.60507/FK2/OM25XQ","metadataLanguage":"en","datasetType":"dataset","datasetVersion":{"id":405,"datasetId":11321,"datasetPersistentId":"doi:10.60507/FK2/OM25XQ","datasetType":"dataset","storageIdentifier":"file://10.60507/FK2/OM25XQ","versionNumber":1,"internalVersionNumber":32,"versionMinorNumber":0,"versionState":"RELEASED","latestVersionPublishingState":"RELEASED","deaccessionLink":"","productionDate":"2025-04-09","lastUpdateTime":"2026-05-12T11:04:18Z","releaseTime":"2026-05-12T11:04:18Z","createTime":"2025-04-14T23:16:00Z","publicationDate":"2026-05-12","citationDate":"2026-05-12","license":{"name":"CC BY 4.0","uri":"http://creativecommons.org/licenses/by/4.0","iconUri":"https://licensebuttons.net/l/by/4.0/88x31.png"},"fileAccessRequest":true,"metadataBlocks":{"citation":{"displayName":"Citation Metadata","name":"citation","fields":[{"typeName":"title","multiple":false,"typeClass":"primitive","value":"Microscopic analysis of human ASC-derived kidney organoids for polycystic kidney disease modelling"},{"typeName":"author","multiple":true,"typeClass":"compound","value":[{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Ashraf, Arsila Palliyulla Kariat"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"University of Bonn"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"https://orcid.org/0009-0008-9590-4323","expandedvalue":{"personName":"Palliyulla Kariat, Arsila Ashraf","@id":"https://orcid.org/0009-0008-9590-4323","scheme":"ORCID","@type":"https://schema.org/Person"}}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Rohe, Jana"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"University of Bonn"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"https://orcid.org/0009-0002-0343-7577","expandedvalue":{"personName":"Rohe, Jana","@id":"https://orcid.org/0009-0002-0343-7577","scheme":"ORCID","@type":"https://schema.org/Person"}}}]},{"typeName":"datasetContact","multiple":true,"typeClass":"compound","value":[{"datasetContactName":{"typeName":"datasetContactName","multiple":false,"typeClass":"primitive","value":"Ashraf, Arsila Palliyulla Kariat"},"datasetContactAffiliation":{"typeName":"datasetContactAffiliation","multiple":false,"typeClass":"primitive","value":"University of Bonn"},"datasetContactEmail":{"typeName":"datasetContactEmail","multiple":false,"typeClass":"primitive","value":"arsilash@uni-bonn.de"}}]},{"typeName":"dsDescription","multiple":true,"typeClass":"compound","value":[{"dsDescriptionValue":{"typeName":"dsDescriptionValue","multiple":false,"typeClass":"primitive","value":"Polycystic kidney disease (PKD) is a genetic disorder and a major contributor to end-stage renal disease. Elucidating the cellular mechanisms underlying PKD necessitates three-dimensional models that accurately replicate cystogenesis in vitro. Existing organoid models frequently exhibit limited expression of adult kidney epithelial markers and lack standardized methods for cyst identification.\n\nThis microscopy imaging data deposit supports our efforts to develop and characterize a model that more faithfully reflects adult kidney epithelium and provides a robust read-out for PKD research. We generated adult stem cell (ASC)-derived human kidney organoids (tubuloids) from patient tissue, and cultured them either in suspension or embedded in extracellular matrix (domes). This approach allowed us to compare organoid structure, cell polarity, cellular composition, and the representation of kidney epithelium during organoid development. Using pharmacological stimulation and tissue imaging, we demonstrate that these tubuloids reproducibly form cysts in vitro upon chronic cAMP signaling, making these data valuable for PKD investigation.\n \nMicroscopy datasets (image and video files) of fixed and live tubuloids are presented, analyzed for cyst formation, expression of specific proteins of interest, and transduction efficiency."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2025-04-15"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"subjectRefinement","multiple":true,"typeClass":"compound","value":[{"subjectRefinementValue":{"typeName":"subjectRefinementValue","multiple":false,"typeClass":"primitive","value":"Basic Research in Biology and Medicine: Cell Biology [201-03]"}}]},{"typeName":"freeKeyword","multiple":true,"typeClass":"compound","value":[{"freeKeywordValue":{"typeName":"freeKeywordValue","multiple":false,"typeClass":"primitive","value":"ADPKD"}},{"freeKeywordValue":{"typeName":"freeKeywordValue","multiple":false,"typeClass":"primitive","value":"cAMP signalling"}},{"freeKeywordValue":{"typeName":"freeKeywordValue","multiple":false,"typeClass":"primitive","value":"Cilia"}},{"freeKeywordValue":{"typeName":"freeKeywordValue","multiple":false,"typeClass":"primitive","value":"Renal cyst"}},{"freeKeywordValue":{"typeName":"freeKeywordValue","multiple":false,"typeClass":"primitive","value":"Tubuloids"}}]},{"typeName":"topicClassification","multiple":true,"typeClass":"compound","value":[{"topicClassKey":{"typeName":"topicClassKey","multiple":false,"typeClass":"primitive","value":"D007668"},"topicClassValue":{"typeName":"topicClassValue","multiple":false,"typeClass":"primitive","value":"Kidney"},"topicClassVocab":{"typeName":"topicClassVocab","multiple":false,"typeClass":"primitive","value":"MeSH"},"topicClassVocabURI":{"typeName":"topicClassVocabURI","multiple":false,"typeClass":"primitive","value":"https://meshb.nlm.nih.gov/record/ui?ui=D007668"}},{"topicClassKey":{"typeName":"topicClassKey","multiple":false,"typeClass":"primitive","value":"D003560"},"topicClassValue":{"typeName":"topicClassValue","multiple":false,"typeClass":"primitive","value":"Cysts"},"topicClassVocab":{"typeName":"topicClassVocab","multiple":false,"typeClass":"primitive","value":"MeSH"},"topicClassVocabURI":{"typeName":"topicClassVocabURI","multiple":false,"typeClass":"primitive","value":"https://meshb.nlm.nih.gov/record/ui?ui=D003560"}},{"topicClassKey":{"typeName":"topicClassKey","multiple":false,"typeClass":"primitive","value":"D002923"},"topicClassValue":{"typeName":"topicClassValue","multiple":false,"typeClass":"primitive","value":"Cilia"},"topicClassVocab":{"typeName":"topicClassVocab","multiple":false,"typeClass":"primitive","value":"MeSH"},"topicClassVocabURI":{"typeName":"topicClassVocabURI","multiple":false,"typeClass":"primitive","value":"https://meshb.nlm.nih.gov/record/ui?ui=D002923"}},{"topicClassKey":{"typeName":"topicClassKey","multiple":false,"typeClass":"primitive","value":"D009940"},"topicClassValue":{"typeName":"topicClassValue","multiple":false,"typeClass":"primitive","value":"Organoids"},"topicClassVocab":{"typeName":"topicClassVocab","multiple":false,"typeClass":"primitive","value":"MeSH"},"topicClassVocabURI":{"typeName":"topicClassVocabURI","multiple":false,"typeClass":"primitive","value":"https://meshb.nlm.nih.gov/record/ui?ui=D009940"}},{"topicClassKey":{"typeName":"topicClassKey","multiple":false,"typeClass":"primitive","value":"D004848"},"topicClassValue":{"typeName":"topicClassValue","multiple":false,"typeClass":"primitive","value":"Epithelium"},"topicClassVocab":{"typeName":"topicClassVocab","multiple":false,"typeClass":"primitive","value":"MeSH"},"topicClassVocabURI":{"typeName":"topicClassVocabURI","multiple":false,"typeClass":"primitive","value":"https://meshb.nlm.nih.gov/record/ui?ui=D004848"}},{"topicClassKey":{"typeName":"topicClassKey","multiple":false,"typeClass":"primitive","value":"D009398"},"topicClassValue":{"typeName":"topicClassValue","multiple":false,"typeClass":"primitive","value":"Nephrology"},"topicClassVocab":{"typeName":"topicClassVocab","multiple":false,"typeClass":"primitive","value":"MeSH"},"topicClassVocabURI":{"typeName":"topicClassVocabURI","multiple":false,"typeClass":"primitive","value":"https://meshb.nlm.nih.gov/record/ui?ui=D009398"}}]},{"typeName":"publication","multiple":true,"typeClass":"compound","value":[{"publicationRelationType":{"typeName":"publicationRelationType","multiple":false,"typeClass":"controlledVocabulary","value":"IsSupplementTo"},"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"Rohe, J., Ashraf, A.P.K., Kehl, M. et al. Modelling kidney cystogenesis using human kidney tubuloid cultures. BMC Mol and Cell Biol 27, 26 (2026). https://doi.org/10.1186/s12860-026-00591-x"},"publicationIDType":{"typeName":"publicationIDType","multiple":false,"typeClass":"controlledVocabulary","value":"doi"},"publicationIDNumber":{"typeName":"publicationIDNumber","multiple":false,"typeClass":"primitive","value":"10.1186/s12860-026-00591-x"},"publicationURL":{"typeName":"publicationURL","multiple":false,"typeClass":"primitive","value":"https://doi.org/10.1186/s12860-026-00591-x"}}]},{"typeName":"language","multiple":true,"typeClass":"controlledVocabulary","value":["English"]},{"typeName":"producer","multiple":true,"typeClass":"compound","value":[{"producerName":{"typeName":"producerName","multiple":false,"typeClass":"primitive","value":"Ashraf, Arsila Palliyulla Kariat"},"producerAffiliation":{"typeName":"producerAffiliation","multiple":false,"typeClass":"primitive","value":"University of Bonn"},"producerAbbreviation":{"typeName":"producerAbbreviation","multiple":false,"typeClass":"primitive","value":"A.A.P.K."}},{"producerName":{"typeName":"producerName","multiple":false,"typeClass":"primitive","value":"Rohe, Jana"},"producerAffiliation":{"typeName":"producerAffiliation","multiple":false,"typeClass":"primitive","value":"University of Bonn"},"producerAbbreviation":{"typeName":"producerAbbreviation","multiple":false,"typeClass":"primitive","value":"J.R."}},{"producerName":{"typeName":"producerName","multiple":false,"typeClass":"primitive","value":"Wachten, Dagmar"},"producerAffiliation":{"typeName":"producerAffiliation","multiple":false,"typeClass":"primitive","value":"Institute of Innate Immunity, University of Bonn"},"producerAbbreviation":{"typeName":"producerAbbreviation","multiple":false,"typeClass":"primitive","value":"D.W."}},{"producerName":{"typeName":"producerName","multiple":false,"typeClass":"primitive","value":"Toma, Marieta Ioana"},"producerAffiliation":{"typeName":"producerAffiliation","multiple":false,"typeClass":"primitive","value":"Institute of Pathology, Universitätsklinikum Bonn"},"producerAbbreviation":{"typeName":"producerAbbreviation","multiple":false,"typeClass":"primitive","value":"M.I.T."}}]},{"typeName":"productionDate","multiple":false,"typeClass":"primitive","value":"2025-04-09"},{"typeName":"productionPlace","multiple":false,"typeClass":"primitive","value":"Bonn, Germany"},{"typeName":"contributor","multiple":true,"typeClass":"compound","value":[{"contributorType":{"typeName":"contributorType","multiple":false,"typeClass":"controlledVocabulary","value":"Project Leader"},"contributorName":{"typeName":"contributorName","multiple":false,"typeClass":"primitive","value":"Toma, Marieta Ioana"}},{"contributorType":{"typeName":"contributorType","multiple":false,"typeClass":"controlledVocabulary","value":"Project Leader"},"contributorName":{"typeName":"contributorName","multiple":false,"typeClass":"primitive","value":"Wachten, Dagmar"}},{"contributorType":{"typeName":"contributorType","multiple":false,"typeClass":"controlledVocabulary","value":"Hosting Institution"},"contributorName":{"typeName":"contributorName","multiple":false,"typeClass":"primitive","value":"Institute of Innate Immunity, University of Bonn"}},{"contributorName":{"typeName":"contributorName","multiple":false,"typeClass":"primitive","value":"Microscope Core Facility, University of Bonn"}}]},{"typeName":"grantNumber","multiple":true,"typeClass":"compound","value":[{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) \tunder Germany’s Excellence Strategy – EXC2151"},"grantNumberValue":{"typeName":"grantNumberValue","multiple":false,"typeClass":"primitive","value":"390873048"}},{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"FOR5547"},"grantNumberValue":{"typeName":"grantNumberValue","multiple":false,"typeClass":"primitive","value":"503306912"}},{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"WA 3382/8-1"},"grantNumberValue":{"typeName":"grantNumberValue","multiple":false,"typeClass":"primitive","value":"513767027"}},{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"Else Kröner Fresenius Foundation"},"grantNumberValue":{"typeName":"grantNumberValue","multiple":false,"typeClass":"primitive","value":"2021.EKFSE.53"}},{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"University of Bonn - Internal funding"}}]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Ashraf, Arsila Palliyulla Kariat"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2025-04-15"},{"typeName":"dateOfCollection","multiple":true,"typeClass":"compound","value":[{"dateOfCollectionStart":{"typeName":"dateOfCollectionStart","multiple":false,"typeClass":"primitive","value":"2023-09-25"},"dateOfCollectionEnd":{"typeName":"dateOfCollectionEnd","multiple":false,"typeClass":"primitive","value":"2025-04-09"}}]},{"typeName":"kindOfData","multiple":true,"typeClass":"primitive","value":["PNG","TIF","AVI","JPG"]}]},"biomedical":{"displayName":"Life Sciences Metadata","name":"biomedical","fields":[{"typeName":"studyDesignType","multiple":true,"typeClass":"controlledVocabulary","value":["Other"]},{"typeName":"studyOtherDesignType","multiple":true,"typeClass":"primitive","value":["Descriptive/Qualitative Design","Analytical Observational Design"]},{"typeName":"studyFactorType","multiple":true,"typeClass":"controlledVocabulary","value":["Biomarkers","Cell Surface Markers","Passages","Time Point","Treatment Type"]},{"typeName":"studyAssayOrganism","multiple":true,"typeClass":"controlledVocabulary","value":["Homo sapiens"]},{"typeName":"studyAssayMeasurementType","multiple":true,"typeClass":"controlledVocabulary","value":["histology","protein identification"]},{"typeName":"studyAssayTechnologyType","multiple":true,"typeClass":"controlledVocabulary","value":["Other"]},{"typeName":"studyAssayOtherTechnologyType","multiple":true,"typeClass":"primitive","value":["Microscopy imaging"]},{"typeName":"studyAssayPlatform","multiple":true,"typeClass":"controlledVocabulary","value":["Other"]},{"typeName":"studyAssayOtherPlatform","multiple":true,"typeClass":"primitive","value":["Leica DM IRB inverted research microscope in combination with a Nikon DS-U3 Digital Sight Camera Control Unit + Microscope","KEYENCE BZ-X Microscope","Leica Stellaris 8 confocal microscope","Sartorius IncuCyte® S3 Live-Cell Analysis"]}]}},"files":[{"description":"Readme file with information on the image datasets.","label":"README_Rohe-Ashraf-et-al_BMC_Microscopic-analysis-of-human-ASC-derived-kidney-organoids-for-polycystic-kidney-disease-modelling.txt","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":52198,"persistentId":"doi:10.60507/FK2/OM25XQ/UFMMKU","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/UFMMKU","filename":"README_Rohe-Ashraf-et-al_BMC_Microscopic-analysis-of-human-ASC-derived-kidney-organoids-for-polycystic-kidney-disease-modelling.txt","contentType":"text/plain","friendlyType":"Plain Text","filesize":17134,"description":"Readme file with information on the image datasets.","storageIdentifier":"file://19e1bd7d097-fbf6260ce54a","rootDataFileId":-1,"md5":"5e5b83701e49ccb9b33ea26bd8f48d97","checksum":{"type":"MD5","value":"5e5b83701e49ccb9b33ea26bd8f48d97"},"tabularData":false,"creationDate":"2026-05-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Bright-field microscopy images of tubuloids in dome and suspension culture from passages P0 to P5.\n","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-1_Dome-and-Suspension-Tubuloids.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14506,"persistentId":"doi:10.60507/FK2/OM25XQ/0CIDDZ","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/0CIDDZ","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-1_Dome-and-Suspension-Tubuloids.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":26820093,"description":"Bright-field microscopy images of tubuloids in dome and suspension culture from passages P0 to P5.\n","storageIdentifier":"file://19a7848ccf7-e43b17968b68","rootDataFileId":-1,"md5":"6d9ac2c44fc8c5cb5e0abfef14c6b374","checksum":{"type":"MD5","value":"6d9ac2c44fc8c5cb5e0abfef14c6b374"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Bright-field microscopy images of dome and suspension tubuloids at P1 and P3, stained for CK7, CK19 and PAX8. Bright-field microscopy images of dome and suspension tubuloids at P1 and P4, stained for GATA3, CDH1 and CD13.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-2_Immunohistochemistry.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14507,"persistentId":"doi:10.60507/FK2/OM25XQ/MQDDJK","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/MQDDJK","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-2_Immunohistochemistry.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":72711309,"description":"Bright-field microscopy images of dome and suspension tubuloids at P1 and P3, stained for CK7, CK19 and PAX8. Bright-field microscopy images of dome and suspension tubuloids at P1 and P4, stained for GATA3, CDH1 and CD13.","storageIdentifier":"file://19a78492ea4-42cb871a559e","rootDataFileId":-1,"md5":"e31532faa580d8881c322183218f8f65","checksum":{"type":"MD5","value":"e31532faa580d8881c322183218f8f65"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Bright-field microscopy images of dome and suspension tubuloids at P1 and P3/P4, stained for CD24, ATP1A1, CDH16 and TACSTD2.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-3_Immunohistochemistry.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14508,"persistentId":"doi:10.60507/FK2/OM25XQ/G9CKDU","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/G9CKDU","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-3_Immunohistochemistry.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":36506669,"description":"Bright-field microscopy images of dome and suspension tubuloids at P1 and P3/P4, stained for CD24, ATP1A1, CDH16 and TACSTD2.","storageIdentifier":"file://19a784b0654-7aefd4521f3b","rootDataFileId":-1,"md5":"c55f32148b775d8e53fe034ba0749009","checksum":{"type":"MD5","value":"c55f32148b775d8e53fe034ba0749009"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Bright-field microscopy images of Forskolin, DMSO or untreated dome and suspension tubuloids used for the quantification of cyst induction.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-4_Cyst-Induction.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14509,"persistentId":"doi:10.60507/FK2/OM25XQ/U1UEZA","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/U1UEZA","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-4_Cyst-Induction.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":904984408,"description":"Bright-field microscopy images of Forskolin, DMSO or untreated dome and suspension tubuloids used for the quantification of cyst induction.","storageIdentifier":"file://19a784c2fb4-9c54d89a91be","rootDataFileId":-1,"md5":"89dbdd5be4b3d4c4602536f35bca3f70","checksum":{"type":"MD5","value":"89dbdd5be4b3d4c4602536f35bca3f70"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Immunofluorescence images of Forskolin, DMSO or untreated dome and suspension tubuloids used for the quantification of tubuloid epithelial width and ciliary length.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-5_Immunostaining.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14510,"persistentId":"doi:10.60507/FK2/OM25XQ/IV1I0J","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/IV1I0J","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-5_Immunostaining.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":876622654,"description":"Immunofluorescence images of Forskolin, DMSO or untreated dome and suspension tubuloids used for the quantification of tubuloid epithelial width and ciliary length.","storageIdentifier":"file://19a784d3f81-f17e98316f00","rootDataFileId":-1,"md5":"78a3ee1be00f93a434e6f0d41d5d61c8","checksum":{"type":"MD5","value":"78a3ee1be00f93a434e6f0d41d5d61c8"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Bright-field microscopy images of live dome tubuloids at P6 to P8 and primary human kidney cells immediately cultured in suspension after tissue dissociation. \nMicroscopy images and slide scans of fixed dome and suspension tubuloids at P1, stained for MIB1.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-S1_Dome-and-Suspension-culture_MIB1.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14511,"persistentId":"doi:10.60507/FK2/OM25XQ/TVT9O2","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/TVT9O2","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-S1_Dome-and-Suspension-culture_MIB1.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":107408209,"description":"Bright-field microscopy images of live dome tubuloids at P6 to P8 and primary human kidney cells immediately cultured in suspension after tissue dissociation. \nMicroscopy images and slide scans of fixed dome and suspension tubuloids at P1, stained for MIB1.","storageIdentifier":"file://19a784ddebe-f61ce2d3d21d","rootDataFileId":-1,"md5":"0572bbc7020d9fa3ba69169e454ce914","checksum":{"type":"MD5","value":"0572bbc7020d9fa3ba69169e454ce914"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Microscopy images and slide scans of fixed dome and suspension tubuloids in P1 and P4, stained for GATA3 and used for quantification.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-S3B_GATA3-quantification.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14512,"persistentId":"doi:10.60507/FK2/OM25XQ/JV4GAD","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/JV4GAD","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-S3B_GATA3-quantification.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":35316732,"description":"Microscopy images and slide scans of fixed dome and suspension tubuloids in P1 and P4, stained for GATA3 and used for quantification.","storageIdentifier":"file://19a784e9fdb-22d5a18eca25","rootDataFileId":-1,"md5":"ef079097875051bf05a402ef76cfa32f","checksum":{"type":"MD5","value":"ef079097875051bf05a402ef76cfa32f"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Immunofluorescence images of rAAV2/8-CMV-EGFP transduced dome and suspension tubuloids at P3D10.","label":"Rohe-Ashraf-et-al-2025-BMC_Figure-S5_Transduction.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":14513,"persistentId":"doi:10.60507/FK2/OM25XQ/QT1BWY","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/QT1BWY","filename":"Rohe-Ashraf-et-al-2025-BMC_Figure-S5_Transduction.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":6480588,"description":"Immunofluorescence images of rAAV2/8-CMV-EGFP transduced dome and suspension tubuloids at P3D10.","storageIdentifier":"file://19a78501154-63b771d6ece6","rootDataFileId":-1,"md5":"d9e7c5a71221c4f871cb7841ba2404db","checksum":{"type":"MD5","value":"d9e7c5a71221c4f871cb7841ba2404db"},"tabularData":false,"creationDate":"2025-11-12","publicationDate":"2026-05-12","fileAccessRequest":true}},{"description":"Phase-contrast and fluorescent microscopy images of live suspension tubuloids at P3, stained with Calcein-AM.\n","label":"Rohe-Ashraf-et-al-2025-BMC_Live-cell-imaging.zip","restricted":false,"version":1,"datasetVersionId":405,"dataFile":{"id":11323,"persistentId":"doi:10.60507/FK2/OM25XQ/QMESYN","pidURL":"https://doi.org/10.60507/FK2/OM25XQ/QMESYN","filename":"Rohe-Ashraf-et-al-2025-BMC_Live-cell-imaging.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":9509076,"description":"Phase-contrast and fluorescent microscopy images of live suspension tubuloids at P3, stained with Calcein-AM.\n","storageIdentifier":"file://1963690115f-a37d5800b796","rootDataFileId":-1,"md5":"a2268a907153af028653f55cb1b81406","checksum":{"type":"MD5","value":"a2268a907153af028653f55cb1b81406"},"tabularData":false,"creationDate":"2025-04-15","publicationDate":"2026-05-12","fileAccessRequest":true}}],"citation":"Ashraf, Arsila Palliyulla Kariat; Rohe, Jana, 2026, \"Microscopic analysis of human ASC-derived kidney organoids for polycystic kidney disease modelling\", https://doi.org/10.60507/FK2/OM25XQ, bonndata, V1"}}