10.60507/FK2/OM25XQ Ashraf, Arsila Palliyulla Kariat Arsila Palliyulla Kariat Ashraf https://orcid.org/0009-0008-9590-4323 University of Bonn Rohe, Jana Jana Rohe https://orcid.org/0009-0002-0343-7577 University of Bonn bonndata 2026 Medicine, Health and Life Sciences Kidney Cysts Cilia Organoids Epithelium Nephrology Ashraf, Arsila Palliyulla Kariat Arsila Palliyulla Kariat Ashraf University of Bonn Rohe, Jana Jana Rohe University of Bonn Wachten, Dagmar Dagmar Wachten Institute of Innate Immunity, University of Bonn Toma, Marieta Ioana Marieta Ioana Toma Institute of Pathology, Universitätsklinikum Bonn Ashraf, Arsila Palliyulla Kariat Arsila Palliyulla Kariat Ashraf University of Bonn Toma, Marieta Ioana Marieta Ioana Toma Wachten, Dagmar Dagmar Wachten Institute of Innate Immunity, University of Bonn Microscope Core Facility, University of Bonn 2025-04-09 2025-04-15 2026-05-12 2023-09-25/2025-04-09 PNG;TIF;AVI;JPG 10.1186/S12860-026-00591-X 10.60507/FK2/OM25XQ/UFMMKU 10.60507/FK2/OM25XQ/0CIDDZ 10.60507/FK2/OM25XQ/MQDDJK 10.60507/FK2/OM25XQ/G9CKDU 10.60507/FK2/OM25XQ/U1UEZA 10.60507/FK2/OM25XQ/IV1I0J 10.60507/FK2/OM25XQ/TVT9O2 10.60507/FK2/OM25XQ/JV4GAD 10.60507/FK2/OM25XQ/QT1BWY 10.60507/FK2/OM25XQ/QMESYN 9509076 26820093 72711309 36506669 904984408 876622654 107408209 35316732 6480588 17134 application/zip application/zip application/zip application/zip application/zip application/zip application/zip application/zip application/zip text/plain 1.0 Creative Commons Attribution 4.0 International License. Polycystic kidney disease (PKD) is a genetic disorder and a major contributor to end-stage renal disease. Elucidating the cellular mechanisms underlying PKD necessitates three-dimensional models that accurately replicate cystogenesis in vitro. Existing organoid models frequently exhibit limited expression of adult kidney epithelial markers and lack standardized methods for cyst identification. This microscopy imaging data deposit supports our efforts to develop and characterize a model that more faithfully reflects adult kidney epithelium and provides a robust read-out for PKD research. We generated adult stem cell (ASC)-derived human kidney organoids (tubuloids) from patient tissue, and cultured them either in suspension or embedded in extracellular matrix (domes). This approach allowed us to compare organoid structure, cell polarity, cellular composition, and the representation of kidney epithelium during organoid development. Using pharmacological stimulation and tissue imaging, we demonstrate that these tubuloids reproducibly form cysts in vitro upon chronic cAMP signaling, making these data valuable for PKD investigation. Microscopy datasets (image and video files) of fixed and live tubuloids are presented, analyzed for cyst formation, expression of specific proteins of interest, and transduction efficiency. Bonn, Germany Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC2151 390873048 FOR5547 503306912 WA 3382/8-1 513767027 Else Kröner Fresenius Foundation 2021.EKFSE.53 University of Bonn - Internal funding