Back to dataset: Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Figure 18A. Analysis of time-lapse confocal images of microglia in 2D and 3D culture

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Dataset: Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'
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Checksum: MD5 da28ef81ccb86b28a85f38bff685c5c1
License: CC BY 4.0

Representative confocal time-lapse images of microglia culture in 2D culture over 10 minutes. We investigated microglial motility in 3D culture. To achieve this, we obtained time-lapse images of microglia using a high-resolution spinning disk confocal microscope equipped with a 60x water immersion objective. A time frame of 30s per image over 10 min with a z-slice interval of 0.5 µm was used to record the motility of microglia in 2D and 3D cultures. We observed that microglia in 3D culture showed a ramified morphology with constant extensions and retractions of processes reminiscent of in vivo-like microglial surveillance (Figure 18A and B). In contrast, microglia in 2D culture showed an amoeboid morphology with membrane ruffling (Figure 18A ).

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Download Figure 18A. Analysis of time-lapse confocal images of microglia in 2D and 3D culture

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