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Thumbnail for Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'

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Persistent identifier
doi:10.60507/FK2/FFPGJA
Published version
1.0
Publication date
2025-03-25
License
CC BY 4.0

Description

Microglia are innate immune cells of the central nervous system. They possess a diverse range of morphological features that enable them to perform different functions, such as surveillance, phagocytosis and immune response. In response to changes in physiological conditions within the CNS, microglia actively alter their shape in order to maintain brain homeostasis. Previous studies on the morphology and function of microglia have been conducted in 2D cell culture systems, which have been associated with an artificial morphology in microglia and altered microglial functions. Consequently, there is an increasing imperative to develop 3D cell culture systems that can more accurately replicate the complex microenvironment of the brain. The present study aims to investigate the impact of defined 3D cell culture conditions on gene expression, morphology, cell motility, innate immune response, and electrophysiological properties of cultured primary murine microglia. To this end, live cell confocal microscopy imaging was employed to visualise microglia within a 3D cell culture environment, thereby enabling the study of their motility, morphology and phagocytosis.The findings of this study demonstrate that microglia develop a more in vivo-like morphology and surveillance motility when cultured under 3D compared to 2D conditions.

Creators

Keywords

Microglia, 3D culture, ECM

Files

Before downloading: Use and redistribution are governed by CC BY 4.0. Review and comply with the license before using the data.
FileTypeBytesChecksum
Figure 18A. Analysis of time-lapse confocal images of microglia in 2D and 3D culturevideo/quicktime526280MD5 da28ef81ccb86b28a85f38bff685c5c1
Figure 18B. Analysis of time-lapse confocal images of microglia in 2D and 3D culturevideo/quicktime199286MD5 3693b3f6aedba7d1152324505df772ee
Figure 21B. Microglial phagocytic assay in 3D culturevideo/quicktime17695423MD5 cf58858d80c87bf778f197cecad9ca9f
Figure 22A. Microglia motility during phagocytosis. video/quicktime4539074MD5 281acbba92f54ef6ea1462c30e645691
Figure 22B. Microglia motility during phagocytosis. video/quicktime4397550MD5 ec83c7531f9c27d54e1328692b923c5a
Bertrand_Tambe_3D_microglia_readme.txttext/plain6228MD5 e05ce1f30c6e5bf0525a5653dbcd73ad
Figure 21A. Microglial phagocytic assay in 3D culturevideo/quicktime18373685MD5 e8a3cc505403ef939f648cd29b7cd72b
Figure 17A. Microglial cell-matrix interaction in 3D culture.video/quicktime3741289MD5 f876f14728e512fd379910e9eb145f3d

Citation

Tambe, Bertrand Agbor, 2025-03-25, Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia', doi:10.60507/FK2/FFPGJA, V1.0

Additional Dataverse fields

Id397
Dataset Typedataset
Internal Version Number30
Latest Version Publishing StateRELEASED
Deaccession LinkNot supplied
Release Time2025-03-25T07:31:58Z
Create Time2025-03-24T21:28:57Z
Citation Date2025-03-25
File Access RequestTrue

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Citation Metadata

Title title
Confocal live cell images of microglia in 3D cell culture from the dissertation ' 3D culture conditions instruct an in vivo-like phenotype in primary microgia'
Author author
  1. Author Name
    Tambe, Bertrand Agbor
    Author Affiliation
    University of Bonn
Dataset Contact datasetContact
  1. Dataset Contact Name
    Tambe, Bertrand Agbor
    Dataset Contact Affiliation
    University of Bonn
    Dataset Contact Email
    s6betamb@uni-bonn.de
Ds Description dsDescription
  1. Ds Description Value
    Microglia are innate immune cells of the central nervous system. They possess a diverse range of morphological features that enable them to perform different functions, such as surveillance, phagocytosis and immune response. In response to changes in physiological conditions within the CNS, microglia actively alter their shape in order to maintain brain homeostasis. Previous studies on the morphology and function of microglia have been conducted in 2D cell culture systems, which have been associated with an artificial morphology in microglia and altered microglial functions. Consequently, there is an increasing imperative to develop 3D cell culture systems that can more accurately replicate the complex microenvironment of the brain. The present study aims to investigate the impact of defined 3D cell culture conditions on gene expression, morphology, cell motility, innate immune response, and electrophysiological properties of cultured primary murine microglia. To this end, live cell confocal microscopy imaging was employed to visualise microglia within a 3D cell culture environment, thereby enabling the study of their motility, morphology and phagocytosis.The findings of this study demonstrate that microglia develop a more in vivo-like morphology and surveillance motility when cultured under 3D compared to 2D conditions.
    Ds Description Date
    2025-03-24
Subject subject
  1. Medicine, Health and Life Sciences
Free Keyword freeKeyword
  1. Free Keyword Value
    Microglia, 3D culture, ECM
Publication publication
  1. Publication Citation
    3D culture conditions instruct an in vivo-like phenotype in primary microglia, Bertrand Agbor Tambe, Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn, 2025.
    Publication IDType
    urn
    Publication IDNumber
    urn:nbn:de:hbz:5-81722
    Publication URL
    https://nbn-resolving.org/urn:nbn:de:hbz:5-81722
Depositor depositor
Tambe, Bertrand
Date Of Deposit dateOfDeposit
2025-03-24

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