10.60507/FK2/9TSLYB Albert Miguel-Lopez Albert Miguel-Lopez University of Bonn Negar Nikbahkt Negar Nikbahkt University of Bonn Carlos Wert Carvajal Carlos Wert Carvajal https://orcid.org/0000-0002-5340-8275 University of Bonn Lena Johanna Gschossmann Lena Johanna Gschossmann University of Bonn Martin Pofahl Martin Pofahl University of Bonn Heinz Beck Heinz Beck https://orcid.org/0000-0002-8961-998X University of Bonn Tatjana Tchumatchenko Tatjana Tchumatchenko https://orcid.org/0000-0001-9137-809X University of Bonn bonndata 2025 Medicine, Health and Life Sciences Tchumatchenko, Tatjana Tatjana Tchumatchenko University of Bonn Albert Miquel Lopez Albert Miquel Lopez Negar Nikbahkt Negar Nikbahkt Heinz Beck Heinz Beck Tatjana Tchumatchenko Tatjana Tchumatchenko 2025-07-29 2025-07-30 10.1101/2025.03.04.641439v1 10.60507/FK2/9TSLYB/MS83EG 10.60507/FK2/9TSLYB/Z1VBLK 10.60507/FK2/9TSLYB/MDWG6M 10.60507/FK2/9TSLYB/006BHQ 10.60507/FK2/9TSLYB/PHRG55 10.60507/FK2/9TSLYB/J8A94D 10.60507/FK2/9TSLYB/RGM29J 10.60507/FK2/9TSLYB/2OEWUH 10.60507/FK2/9TSLYB/HUFO3Q 10.60507/FK2/9TSLYB/SRYOZL 10.60507/FK2/9TSLYB/5QIE9K 10.60507/FK2/9TSLYB/64VA7D 10.60507/FK2/9TSLYB/KXXBT2 10.60507/FK2/9TSLYB/53IPNT 14690 24730 36038 437003 1727 8799 247626 5959 685 272868 16429 52820 18049 4698 application/octet-stream text/x-python application/octet-stream application/octet-stream text/markdown text/x-python text/x-python application/octet-stream application/octet-stream application/octet-stream application/octet-stream text/x-python text/x-python text/plain 1.0 Creative Commons Attribution 4.0 International License. Hippocampal circuits form cognitive maps representing spatial position and integrating contextual information, including affective cues, within episodic memory representations. We investigated how spatial and affective information combine in the population activity of CA3 axons by imaging intermediate-to-dorsal and dorsal-to-dorsal projections in mice navigating a linear track before, during, and after exposure to an aversive air puff stimulus. Our analyses reveal that both axonal populations maintain a robust, time-invariant spatial coding manifold across recordings, independent of affective context. Alterations to this common manifold encode the presence of the aversive stimulus without disrupting the spatial representation. Both axonal pathways encoded affective information with similar efficacy. This population-level encoding was distributed similarly across place and non-place cells. Our findings demonstrate that hippocampal CA3 axons integrate spatial and affective information within a common representational geometry while preserving the ability to extract each information type separately. Deutsche Forschungsgemeinschaft DFG