10.60507/FK2/4KUKXIHorz, JakobJakobHorz0009-0007-7367-9439University of BonnWolff, KatharinaKatharinaWolff0000-0002-3616-0482University of BonnFriedhoff, RonjaRonjaFriedhoff0009-0002-0270-4661TU BraunschweigPucker, BoasBoasPucker0000-0002-3321-7471University of Bonnbonndata2025Medicine, Health and Life SciencesPucker, BoasUniversity of Bonn2025-10-122026-02-1510.1101/2024.02.14.5803031787303211509650987469152657212423259751006598334927345477application/gzipapplication/gzipapplication/gzipapplication/gzipapplication/gzipapplication/gzipapplication/gziptext/plain2.0CC BY 4.0Here we present a high-quality genome sequence and annotation of Digitalis purpurea (commonly known as foxglove), a plant valued both for its ornamental flowers and for producing the cardiac drug digoxin. The study aims to uncover the genetic basis of flower color variation in this species. Using ONT long-read sequencing data, we assembled the genome sequence of a magenta-flowering D. purpurea individual. We performed extensive gene prediction and functional annotation of protein-coding genes. Genes of the flavonoid biosynthesis were compared between magenta and white flowering individuals. Expression patterns were compared between different plant organs and between plants with different flower colors. Through these analyses, we identified a large insertion in the anthocyanidin synthase (ANS) that explains the differences between magenta and white flowers. This data publication contains the genome sequence with the corresponding annotation V2.