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Clinical dataset on monocyte cholesterol crystal uptake and cyclodextrin exposure in patients undergoing coronary angiography

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Important usage condition: Use and redistribution are governed by CC BY 4.0. Review and comply with the license before using the data.
Persistent identifier
doi:10.60507/FK2/0SYROH
Published version
1.0
Publication date
2025-12-21
License
CC BY 4.0

Description

This observational study includes patients undergoing diagnostic coronary angiography. For each participant we document demographic characteristics, cardiovascular risk factors, angiographic findings (presence and extent of CAD, PCI), laboratory markers of inflammation and metabolism, and medication at the time of the study. In addition, we assess cholesterol crystal (CC) uptake by circulating monocytes and the corresponding cyclodextrin (CD)-related effect (ΔCC) as imaging-/signal–derived measures. The overall purpose is to characterize this patient cohort and to examine whether CC uptake and its change are related to the burden of coronary artery disease and to clinically relevant parameters such as hypertension, diabetes mellitus, lipid profile, inflammatory markers (e.g. CRP, leukocytes) and current cardiovascular medication.

Creators

Keywords

cholesterol crystal uptake

Files

Before downloading: Use and redistribution are governed by CC BY 4.0. Review and comply with the license before using the data.
FileTypeBytesChecksum
S1_Data_DataDictionary.csvtext/csv3528MD5 9aea0e3d82f45c7b9ff66b5a8d599720
S1_Data.csvtext/csv7224MD5 6a92d54a4b6f24fc86a3a8e7e29fe09e
_Readme.txttext/plain10482MD5 a18be75667202550b0913087d98f4ffa

Citation

Luebbering, Nikola; Zimmer, Sebastian, 2025-12-21, Clinical dataset on monocyte cholesterol crystal uptake and cyclodextrin exposure in patients undergoing coronary angiography, doi:10.60507/FK2/0SYROH, V1.0

Additional Dataverse fields

Id521
Dataset Typedataset
Internal Version Number20
Latest Version Publishing StateRELEASED
Release Time2025-12-21T18:27:53Z
Create Time2025-11-11T16:50:52Z
Citation Date2025-12-21
File Access RequestTrue

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Complete Dataverse metadata

Citation Metadata

Title title
Clinical dataset on monocyte cholesterol crystal uptake and cyclodextrin exposure in patients undergoing coronary angiography
Author author
  1. Author Name
    Luebbering, Nikola
    Author Affiliation
    University of Bonn
    Author Identifier Scheme
    ORCID
    Author Identifier
    Type Name
    authorIdentifier
    Multiple
    False
    Type Class
    primitive
    Value
    https://orcid.org/0009-0003-6239-2919
    Expandedvalue
    Person Name
    Luebbering, Nikola
    @id
    https://orcid.org/0009-0003-6239-2919
    Scheme
    ORCID
    @type
    https://schema.org/Person
  2. Author Name
    Zimmer, Sebastian
    Author Affiliation
    University of Bonn
    Author Identifier Scheme
    ORCID
    Author Identifier
    Type Name
    authorIdentifier
    Multiple
    False
    Type Class
    primitive
    Value
    https://orcid.org/0000-0002-1531-2088
    Expandedvalue
    Person Name
    Zimmer, Sebastian
    @id
    https://orcid.org/0000-0002-1531-2088
    Scheme
    ORCID
    @type
    https://schema.org/Person
Dataset Contact datasetContact
  1. Dataset Contact Name
    Lübbering, Nikola
    Dataset Contact Affiliation
    University of Bonn
    Dataset Contact Email
    nlue@uni-bonn.de
Ds Description dsDescription
  1. Ds Description Value
    This observational study includes patients undergoing diagnostic coronary angiography. For each participant we document demographic characteristics, cardiovascular risk factors, angiographic findings (presence and extent of CAD, PCI), laboratory markers of inflammation and metabolism, and medication at the time of the study. In addition, we assess cholesterol crystal (CC) uptake by circulating monocytes and the corresponding cyclodextrin (CD)-related effect (ΔCC) as imaging-/signal–derived measures. The overall purpose is to characterize this patient cohort and to examine whether CC uptake and its change are related to the burden of coronary artery disease and to clinically relevant parameters such as hypertension, diabetes mellitus, lipid profile, inflammatory markers (e.g. CRP, leukocytes) and current cardiovascular medication.
Subject subject
  1. Medicine, Health and Life Sciences
Free Keyword freeKeyword
  1. Free Keyword Value
    cholesterol crystal uptake
Topic Classification topicClassification
  1. Topic Class Key
    D001018
    Topic Class Value
    Aortic Diseases
    Topic Class Vocab
    MeSH
    Topic Class Vocab URI
    https://id.nlm.nih.gov/mesh/D001018.html
  2. Topic Class Key
    D009000
    Topic Class Value
    Monocytes
    Topic Class Vocab
    Mesh
    Topic Class Vocab URI
    https://id.nlm.nih.gov/mesh/D009000.html
  3. Topic Class Key
    D002784
    Topic Class Value
    Cholesterol
    Topic Class Vocab
    MeSH
    Topic Class Vocab URI
    https://id.nlm.nih.gov/mesh/D002784.html
  4. Topic Class Key
    D003505
    Topic Class Value
    Cyclodextrins
    Topic Class Vocab
    MeSH
    Topic Class Vocab URI
    https://id.nlm.nih.gov/mesh/D003505.html
Publication publication
  1. Publication Relation Type
    IsSupplementTo
    Publication Citation
    Lübbering N, Krogmann A, Jansen F, Latz E, Nickenig G, Zimmer S (2025) Cyclodextrin reduces cholesterol crystal uptake by circulating monocytes in patients undergoing coronary angiography. PLoS One 20(12): e0338635.
    Publication IDType
    doi
    Publication IDNumber
    10.1371/journal.pone.0338635
    Publication URL
    https://doi.org/10.1371/journal.pone.0338635
Notes Text notesText
Sample collection protocol: Whole blood samples were collected from 76 patients undergoing coronary angiography Sample preparation protocol: For separation of peripheral blood mononuclear cells (PBMC), a cell separation medium with a polyester gel and a density gradient liquid was used. PBMC were dissolved in the patient`s own serum (7.2 ml) and diluted in (7.8 ml) phosphate-buffered saline (PBS). Analysis protocol: The PBMC were preincubated with 2-Hydroxypropyl-gamma-CD (10 mM) or PBS control for 6h at 37°C and subsequently incubated with CC (120 x 106 particles) for 30 min at 37°C. CC-uptake by monocytes was evaluated and quantified by flow cytometry. After stimulation with CD and CC, PBMC were treated with 12.5 µl 4 % paraformaldehyde in PBS. To differentiate monocytes from lymphocytes and cell debris, we used three different monoclonal antibodies: PE anti-human CD11b (Clone ICRF44, BioLegend, San Diego, CA, USA), FITC anti-human CD14 (Clone M5E2, BioLegend) und PerCP anti-human CD45 (Clone 2D1, BioLegend) (Dilution: 1 µg/test). Methods for processing the data: CC uptake was measured using the protocol described above. The CCΔCD value was calculated as the difference between CC uptake without CD and CC uptake with CD.
Language language
  1. English
Production Place productionPlace
University Hospital Bonn
Contributor contributor
  1. Contributor Type
    Researcher
    Contributor Name
    Krogmann, Alexander
  2. Contributor Type
    Researcher
    Contributor Name
    Jansen, Felix
  3. Contributor Type
    Researcher
    Contributor Name
    Latz, Eicke
  4. Contributor Type
    Supervisor
    Contributor Name
    Nickenig, Georg
Grant Number grantNumber
  1. Grant Number Agency
    Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
    Grant Number Value
    397484323 – TRR259 – A02
Depositor depositor
Lübbering, Nikola
Date Of Deposit dateOfDeposit
2025-11-11
Date Of Collection dateOfCollection
  1. Date Of Collection Start
    2017-11-15
    Date Of Collection End
    2018-02-14
Kind Of Data kindOfData
  1. qualitative
  2. quantitative
  3. tabular
Software software
  1. Software Name
    Graph Pad Prism
    Software Version
    7
  2. Software Name
    SPSS Statistics
    Software Version
    25

Life Sciences Metadata

Study Design Type studyDesignType
  1. Cross Sectional
Study Assay Organism studyAssayOrganism
  1. Homo sapiens
Study Assay Measurement Type studyAssayMeasurementType
  1. cell counting
  2. Other
Study Assay Other Measurment Type studyAssayOtherMeasurmentType
  1. cholesterol uptake measurement; anamnesis
Study Assay Technology Type studyAssayTechnologyType
  1. flow cytometry
  2. Other
Study Assay Other Technology Type studyAssayOtherTechnologyType
  1. medical records review

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